Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs

Abstract Background Mesenchymal stem cell treatments in dogs have been investigated as a potential innovative alternative to current conventional therapies for variety of conditions. So far, the precise mode action MSCs has yet be determined. The aim this study was gain more insights into pharmacokinetics by evaluating their biodistribution healthy after different injection routes. Methods Three studies were performed evaluate pattern radiolabelled equine peripheral blood-derived mesenchymal cells following intravenous, intramuscular and subcutaneous administration comparison with free 99m Technetium. labelling using stannous chloride reducing agent. Whole-body scans obtained gamma camera during 24-h follow-up. Results efficiency ranged between 59.58 83.82%. Free Technetium accumulation predominantly observed stomach, thyroid, bladder salivary glands, while intravenous injection, Technetium-labelled majorly accumulated liver throughout follow-up period. After injected dose percentage remained very high at site. Conclusions A distinct difference noted compared indicating specific pharmacokinetic systemic dogs. Furthermore, used xenogeneic appeared from previously reported experiments sources cells.